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. 2016 Jun 10;473(12):1693–1702. doi: 10.1042/BCJ20160092

Figure 6. ncOGT is sufficient for O-GlcNAcylation of mitochondrial proteins.

Figure 6

(A) HEK 293 suspension cells were transfected with either GFP or GFP-tagged ncOGT. Mitochondria, purified from transfected cells, were incubated with buffer or with 10 μg/ml of proteinase K. Lysates from the mitochondrial or the cytosolic fractions were probed with anti-OGT, anti-α-tubulin (cytosol), anti-ATPB (mitochondrial matrix) and anti-TOM20 (mitochondrial outer membrane) antibodies. (B) O-GlcNAc levels in mitochondrial fractions were detected with anti-O-GlcNAc antibody (CTD 110.6). The amount of protein loaded in each lane in (A) is 10 μg, whereas approximately 100 μg was used in (B).