Figure 1. Size distributions of EVs released by human and Drosophila cells.

(a) Histograms depicting the diameter distribution of particles in cell-depleted supernatant, as determined by nanoparticle tracking analyses (Nanosight) for human (H.s.) and Drosophila (D.m.) cells. Mean (μ) and standard deviation (σ) with associated standard error measurements (s.e.m) are overlaid on histograms. (b) Representative transmission electron micrographs of human HepG2 (left panels) and Drosophila D17 (right panels) EVs purified from culture supernatants and stained with uranyl acetate. Scale bars = 50 nm. (c) Whisker plot of diameter distributions of HepG2 (left) and D17 (right) EVs, as determined by direct quantification of electronic micrographs. The number (n) of EVs quantified is indicated.