Table 2.
Primers used in this study
| Primer | Sequence (5′ → 3′)a | Description and location |
|---|---|---|
| Primer 1 | ATCCATGGGTATGAAAAAAAAGATTAT | Upstream of SPP2-F, NcoIb |
| Primer 2 | CATGCATGCTGCGTCACAAGTCGACGATA | Downstream of SPP2-R, SphI |
| Primer 3 | CATGCATGCATGAACTCACTGGTTTCTT | Upstream of kiss1-F, SphIc |
| Primer 4 | CCCAAGCTTCAGCCCCGCCCAGCGCTTC | Downstream of kiss1-R, HindIII |
| Primer 5 | ACCACAGTCCATGCCATCAC | Upstream of GAPDH for RT-PCR |
| Primer 6 | TCCACCACCCTGTTGCTGTA | Downstream of GAPDH for RT-PCR |
| Primer 7 | GGT GGA CCG GAT GTT CCC | Upstream of MMP-9 for RT-PCR |
| Primer 8 | GCC CAC CTC TCC TCC | Downstream of MMP-9 for RT-PCR |
aThe added restriction enzyme sites are underlined
bSPP2 includes Usp45 signal peptide and LEISSTCDA synthetic propeptide
c Homo sapiens KiSS-1 metastasis suppressor: gb:BC022819.1 (cDNA clone MGC:39258 IMAGE:5443510)