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. 2016 Jun 10;16:104. doi: 10.1186/s12866-016-0720-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Metabolism of 22:6n-3 and VFA accumulation at different time points by Butyrivibrio proteoclasticus P18. Growth medium included 50 % (v/v) of autoclaved-uncentrifuged rumen fluid. Hydrogen was used as the headspace gas. ● Residual 22:6n-3 in the tube and ▲ VFA accumulated. Results are means and SD from 2 biological replicates, each of which had 2 analytical replicates. For residual 22:6n-3 and accumulated VFA, data points with different letters are significantly different (P < 0.05)