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. 2016 Jun 10;16:104. doi: 10.1186/s12866-016-0720-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Metabolism of 22:6n-3 and optical density measurements at different time points by Butyrivibrio proteoclasticus P18. Growth medium included 20 % (v/v) of autoclaved-centrifuged rumen fluid. Hydrogen was used as the headspace gas. ● Residual 22:6n-3 in the tube ♦ Optical density (OD₆₀₀) measured at respective time points. Results are means and SD from 3 replicates. For residual 22:6n-3 and OD₆₀₀ measured, data points with different letters are significantly different (P < 0.05)