Table 1.

Overview of the in vitro experiments conducted in this study

Exp.	Autoclaved-rumen fluid/tube (%)	Bacteriaa	22:6n-3 (μg/mL)	Gas phaseb	Incubation period (h)
	Uncentrifuged
1	20 and 50	B. fibri	20	CO2	0 and 48
2	50	B. proteo	20	CO2, H2 and N2	0 and 48
3	50	B. proteo	20	H2	0, 2, 4, 8, 12, 24 and 48
4	50	B. proteo	5, 10, 40 and 80	H2	0 and 48
5c	50	B. proteo	10 and 40	H2	0, 2, 4, 8, 12, 24 and 48
	Centrifuged
6	20 and 50	B. fibri	0.5, 1, 2, 4, 8, 16 and 32	CO2	0 and 48
7	20	B. proteo	20	H2	0 and 24
8	20	B. proteo	20	H2	0, 2, 4, 8, 12 and 24

^aBacteria used in the experiment -B. fibri: Butyrivibrio fibrisolvens D1; B. proteo: Butyrivibrio proteoclasticus P18

^bheadspace gas phase

^cThe growth medium used in experiment 5 contained 40 μg/mL of 18:2n-6