Table 3.
Amount of metabolized 22:6n-3 (μg/tube) recovered in FAME peaks. Incubation was performed in growth medium containing autoclaved-uncentrifuged rumen fluid (50 % v/v) in the presence of 22:6n-3 (20 μg/mL) with Butyrivibrio proteoclasticus P18. Tubes were withdrawn at 0, 2, 4, 8, 12, 24 and 48 h incubation to study the extent of 22:6n-3 biohydrogenation. Peak numbers correspond to peaks in Fig. 2
| Peaks | Incubation duration (h) | |||||
|---|---|---|---|---|---|---|
| 4 | 8 | 12 | 24 | 48 | ||
| 1 | Δ7, Δ10, Δ13, Δ16, Δ19-22:5 | 2.18 ± 0.66 | 16.88 ± 0.94 | 16.38 ± 2.09 | 3.39 ± 1.71 | 2.93 ± 1.04 |
| 2 | Δ4, Δ10, Δ13, Δ16, Δ19-22:5 | - | 3.36 ± 0.38 | 4.19 ± 1.04 | 1.07 ± 0.34 | 0.43 ± 0.58 |
| 3 | Δ5, Δ10, Δ13, Δ16, Δ19-22:5 | 3.92 ± 1.29 | 44.38 ± 1.59 | 16.60 ± 5.80 | 7.40 ± 3.51 | 3.68 ± 2.42 |
| 4 | Δ10, Δ13, Δ16, Δ19-22:4 | 9.42 ± 2.55 | 55.40 ± 3.00 | 11.27 ± 2.34 | 19.69 ± 2.41 | |
| 5 | Δ13, Δ16, Δ20-22:3 | 3.12 ± 0.70 | 8.17 ± 1.99 | 1.73 ± 0.11 | 3.63 ± 1.62 | |
| 6 | Δ13, Δ16, Δ19-22:3 | - | 9.91 ± 1.90 | 33.05 ± 4.66 | 36.02 ± 5.88 | |
| 7 | Δ13, Δ16, Δ20-22:3 | - | 0.69 ± 0.86 | 4.91 ± 0.38 | 7.24 ± 0.49 | |
| 8 | Δ11, Δ16, Δ19-22:3 | - | 9.10 ± 0.99 | 10.69 ± 1.66 | 8.77 ± 1.22 | |
| 9 | Δ16, Δ20-22:2 | - | 0.72 ± 0.90 | 7.45 ± 0.49 | 10.75 ± 0.76 | |
| 10 | Δ16, Δ19-22:2 | - | 6.31 ± 1.67 | 31.24 ± 2.96 | 37.31 ± 4.61 | |
| 11 | Δ16, Δ20-22:2 | - | 1.34 ± 0.93 | 11.75 ± 1.25 | 16.69 ± 1.46 | |
| 12 | Δ14, Δ18-22:2 | - | - | 5.03 ± 0.48 | 7.49 ± 0.56 | |