Table 5.
Amount of metabolized 22:6n-3 (μg/tube) recovered in FAME peaks. Incubation was performed in growth medium containing autoclaved-centrifuged rumen fluid (20 % v/v) in the presence of 22:6n-3 (20 μg/mL) with Butyrivibrio proteoclasticus P18. Tubes were withdrawn at 0, 2, 4, 8, 12 and 24 h incubation to study the extent of 22:6n-3 biohydrogenation. Peak numbers correspond to peaks in Fig. 2
| Peaks | Incubation duration (h) | ||||
|---|---|---|---|---|---|
| 4 | 8 | 12 | 24 | ||
| 1 | Δ7, Δ10, Δ13, Δ16, Δ19-22:5 | 5.51 ± 0.59 | 19.55 ± 2.82 | 13.72 ± 3.93 | - |
| 2 | Δ4, Δ10, Δ13, Δ16, Δ19-22:5 | - | 3.68 ± 1.22 | 5.21 ± 3.88 | - |
| 3 | Δ5, Δ10, Δ13, Δ16, Δ19-22:5 | 4.41 ± 1.10 | 53.32 ± 8.58 | 28.37 ± 39.9 | - |
| 4 | Δ10, Δ13, Δ16, Δ19-22:4 | 11.34 ± 3.05 | 91.90 ± 40.6 | 26.41 ± 36.1 | |
| 5 | Δ13, Δ16, Δ20-22:3 | 6.89 ± 1.25 | 11.86 ± 3.27 | 9.34 ± 5.26 | |
| 6 | Δ13, Δ16, Δ19-22:3 | - | 10.26 ± 5.80 | 38.69 ± 3.97 | |
| 7 | Δ13, Δ16, Δ20-22:3 | - | 0.36 ± 0.32 | 6.75 ± 5.59 | |
| 8 | Δ11, Δ16, Δ19-22:3 | - | 10.10 ± 6.52 | 15.73 ± 16.6 | |
| 9 | Δ16, Δ20-22:2 | - | 0.34 ± 0.30 | 7.36 ± 7.36 | |
| 10 | Δ16, Δ19-22:2 | - | 1.38 ± 1.21 | 26.68 ± 11.4 | |
| 11 | Δ16, Δ20-22:2 | - | - | 10.09 ± 10.8 | |
| 12 | Δ14, Δ18-22:2 | - | - | 6.69 ± 5.96 | |