Table 6.
Amount of metabolized 22:6n-3 (μg/tube) recovered in FAME peaks. Incubation was performed in growth medium containing autoclaved-uncentrifuged rumen fluid (50 % v/v) in the presence of 22:6n-3 (5, 10, 20, 40 and 80 μg/mL) with Butyrivibrio proteoclasticus P18. Tubes were withdrawn at 48 h of incubation to study the extent of 22:6n-3 biohydrogenation. Peak numbers correspond to peaks in Fig. 2
| Initial concentration of 22:6n-3 (μg/tube) | ||||||
|---|---|---|---|---|---|---|
| Peaks | 50 | 100 | 200 | 400 | 800 | |
| 1 | Δ7, Δ10, Δ13, Δ16, Δ19-22:5 | 0.63 ± 0.75 | 1.15 ± 0.28 | 2.93 ± 1.04 | 25.14 ± 3.03 | 77.118 ± 1.30 |
| 2 | Δ4, Δ10, Δ13, Δ16, Δ19-22:5 | - | - | 0.43 ± 0.58 | 5.08 ± 1.05 | 21.42 ± 0.69 |
| 3 | Δ5, Δ10, Δ13, Δ16, Δ19-22:5 | 0.87 ± 1.13 | 1.11 ± 0.96 | 3.68 ± 2.42 | 29.16 ± 3.92 | 177.29 ± 5.04 |
| 4 | Δ10, Δ13, Δ16, Δ19-22:4 | 5.26 ± 1.98 | 8.14 ± 2.11 | 19.69 ± 2.41 | 208.41 ± 14.7 | 78.81 ± 7.61 |
| 5 | Δ13, Δ16, Δ20-22:3 | 0.41 ± 0.82 | 1.17 ± 0.82 | 3.63 ± 1.62 | 15.80 ± 2.34 | 12.05 ± 1.17 |
| 6 | Δ13, Δ16, Δ19-22:3 | 5.82 ± 0.77 | 14.26 ± 0.62 | 36.02 ± 5.88 | 22.22 ± 3.63 | 3.48 ± 1.19 |
| 7 | Δ13, Δ16, Δ20-22:3 | 2.84 ± 0.46 | 5.02 ± 0.20 | 7.24 ± 0.49 | - | - |
| 8 | Δ11, Δ16, Δ19-22:3 | 2.33 ± 0.86 | 4.26 ± 0.66 | 8.77 ± 1.22 | 26.98 ± 3.47 | 5.61 ± 0.81 |
| 9 | Δ16, Δ20-22:2 | 4.14 ± 0.53 | 7.14 ± 0.39 | 10.75 ± 0.76 | - | - |
| 10 | Δ16, Δ19-22:2 | 7.81 ± 0.81 | 15.92 ± 0.87 | 37.31 ± 4.61 | 5.53 ± 1.24 | 3.09 ± 1.05 |
| 11 | Δ16, Δ20-22:2 | 8.83 ± 0.63 | 12.94 ± 0.59 | 16.69 ± 1.46 | - | - |
| 12 | Δ14, Δ18-22:2 | 3.34 ± 0.65 | 5.09 ± 0.35 | 7.49 ± 0.56 | - | - |