. 2016 Jun 10;16:104. doi: 10.1186/s12866-016-0720-9

Table 7.

Linoleic acid metabolism (mg/tube) in presence of 22:6n-3 by B. proteoclasticus P18. Incubation was performed in growth medium containing 40 μg/mL (0.4 mg/tube) of 18:2n-6 in presence of 0, 10 μg/mL (Low: 0.1 mg/tube) and 40 μg/mL (High: 0.4 mg/tube) 22:6n-3. Growth medium included 50 % (v/v) of autoclaved-uncentrifuged rumen fluid and H₂ gas was the headspace gas. Value-presented in this table included the fatty acids and VFA of autoclaved-uncentrifuged rumen fluid used to prepare the growth medium

	Incubation duration (h)
	22:6n-3	0	2	4	8	12	24	48
18:2n-6	0	0.27 ± 0.006^a	0.09 ± 0.014^b	0.08 ± 0.009^bc	0.08 ± 0.002^bc	0.09 ± 0.012^b	0.06 ± 0.001^c	0.05 ± 0.002^c
(mg/tube)	Low	0.25 ± 0.007^a	0.09 ± 0.001^bc	0.09 ± 0.002^bc	0.09 ± 0.001^bc	0.10 ± 0.003^b	0.07 ± 0.006^c	0.06 ± 0.002^c
	High	0.28 ± 0.099^a	0.11 ± 0.002^b	0.10 ± 0.011^bc	0.10 ± 0.004^b	0.10 ± 0.006^b	0.07 ± 0.006^c	0.06 ± 0.005^c
CLA	0	0.07 ± 0.005^a	0.02 ± 0.007^b	0.00^b	0.00^b	0.00^b	0.00^b	0.00^b
(mg/tube)	Low	0.10 ± 0.007^a*	0.02 ± 0.004^b	0.00^b	0.00^b	0.00^b	0.00^b	0.00^b
	High	0.11 ± 0.073^a	0.02 ± 0.003^b	0.00^b	0.00^b	0.00^b	0.00^b	0.00^b
VA	0	0.05 ± 0.005^a	0.27 ± 0.026^b	0.21 ± 0.011^c	0.07 ± 0.003^d	0.06 ± 0.005^d	0.06 ± 0.003^d	0.04 ± 0.008^a
(mg/tube)	Low	0.05 ± 0.005^a	0.30 ± 0.010^b*	0.29 ± 0.015^c*	0.10 ± 0.007^d*	0.07 ± 0.003^e	0.07 ± 0.004^e	0.07 ± 0.004^e*
	High	0.05 ± 0.026^a	0.29 ± 0.005^b*	0.28 ± 0.005^b*	0.19 ± 0.005^c**	0.11 ± 0.008^d*	0.13 ± 0.010^d*	0.10 ± 0.002^d**
18:0	0	0.50 ± 0.011^a	0.51 ± 0.041^a	0.58 ± 0.014^b	0.74 ± 0.056^c	0.73 ± 0.020^c	0.71 ± 0.016^c	0.73 ± 0.019^c
(mg/tube)	Low	0.53 ± 0.019^a	0.53 ± 0.009^a	0.53 ± 0.022^a*	0.67 ± 0.019^b*	0.69 ± 0.018^bc*	0.72 ± 0.010^c	0.73 ± 0.010^c
	High	0.53 ± 0.006^a	0.53 ± 0.003^a	0.52 ± 0.017^a*	0.54 ± 0.015^a**	0.58 ± 0.015^b**	0.65 ± 0.017^c*	0.68 ± 0.007^c*
VFA	0	434.1 ± 4.79^a	436.4 ± 7.77^a	469.6 ± 16.80^b	541.7 ± 12.61^c	545.7 ± 8.57^c	585.2 ± 30.61^d	567.6 ± 15.93^d
(μmol/tube)	Low	434.0 ± 9.61^a	437.7 ± 6.01^a	460.6 ± 3.09^b	527.9 ± 6.54^cd	542.9 ± 4.34^de	557.0 ± 7.23^e*	551.4 ± 5.35^e
	High	449.1 ± 16.6^a	433.0 ± 8.91^a	468.5 ± 21.71^b	531.8 ± 8.25^c	549.7 ± 6.14^cd	562.1 ± 17.97^d*	559.4 ± 2.82^d
22:6n-3	Low	0.07 ± 0.005^a	0.07 ± 0.002^a	0.06 ± 0.002^b	0.02 ± 0.001^c	0.02 ± 0.001^c	0.02 ± 0.003^c	0.01 ± 0.000^d
(mg/tube)	High	0.31 ± 0.018^a	0.30 ± 0.012^a	0.29 ± 0.011^a	0.17 ± 0.002^b	0.11 ± 0.004^c	0.09 ± 0.012^c	0.06 ± 0.006^d

CLA conjugated linoleic acid, VA vaccenic acid, VFA volatile fatty acids

For each fatty acids, subscript * represents different (P < 0.05) compared to 0 mg/tube 22:6n-3 and ** represents different (P < 0.05) compared to 0 and 0.1 mg/tube 22:6n-3 at the respective time point

Within each row means with different superscripts (a-e) are significantly different (P < 0.05)