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. 2016 Apr 26;31(7):1552–1561. doi: 10.1093/humrep/dew080

Figure 4.

Figure 4

Expression of NOTCH1 and C3 in the endometrium of oocyte donors following intrauterine hCG infusion. (A) Intrauterine hCG did not significantly impact mRNA expression levels of NOTCH1 or C3 by quantitative RT–PCR. (B) Both endometrial glandular epithelial (GE) and stromal compartments from hCG-treated patients expressed significantly elevated levels of NOTCH1 protein. Consistent with our findings in the non-human primate, intrauterine infusion of hCG significantly increased endometrial stromal C3 protein expression while there was no significant impact on C3 expression in the GE. Staining intensity for GE and stromal compartments was quantified by image analysis software ImageJ (D-HSCORE) for statistical analysis. *P < 0.05, **P < 0.01; Scale bar = 100 µm.