Figure 8.

Prospects for novel layouts enabling lab-on-a-chip applications and improved orientation of enzymatically active TMV rods. A: Scheme of a microchannel of a glucose sensor chip equipped with a blend of two TMV enzyme nanocarrier species. By an appropriate mixture thereof, an optimally composed two-enzyme cascade of [SA]-GOx and [SA]-HRP can be established in a PDMS flow cell. B: TMV-like particles mounted in an upright position in a nanowell template fabricated as follows: A flat substrate was equipped with ssDNA and covered with a 60 nm thin, perforated metal-organic film produced by metal polymer blend lithography, followed by a metal-organic build-up reaction. OAs-containing RNA was subjected to hybridization with DNA on the bottom of the holes, then assembly-competent CP was added to yield TMV-like rods within the pores. The AFM Image (left) shows two TMV rods (black and red arrow) protruding from the 60 nm deep holes.