Figure 11. Increased pre-selection formation of disease associated public TCRβ.

To determine the diversity of the public autoimmune repertoire in pre-selection thymocytes, nucleotide (nt) sequences identified for 48 highly public CNS TCRβ amino acid sequences were assessed in sorted pre-selection CD4⁺CD8⁺TCR^lo thymocytes from healthy mice (n=4). The mean number of unique nt sequences per thymus for each of the 48 sequences is plotted. For each CNS sequence, paired control private sequences were identified in each mouse with the same TRBV, TRBJ, and CDR3β length, and similar frequency (>0.25–<4 fold) as the public sequence for comparison. Mean unique nt sequences is similarly plotted.