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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Anal Chem. 2015 Nov 12;87(23):11777–11783. doi: 10.1021/acs.analchem.5b03123

Table 1.

HDX rates for non-interfacial regions at different pH values.

Method 1 pH 3.5
(× 10−3 s−1)		 Method 2 pH 3.5
(× 10−3 s−1)		 Method 1 pH 4
(× 10−3 s−1)		 Method 2 pH 4
(× 10−3 s−1)
Leu1-Thr4 5.4 ± 0.4 3.4 ± 0.2 9.6 ± 1.4 5.1 ± 1.0
Gln5-Met7 3.2 ± 0.3 2.9 ± 0.5 7.4 ± 1.0 3.9 ± 0.5
Lys8-Gln13 5.2 ± 0.6 5.2 ± 0.8 9.3 ± 1.2 10.2 ± 2.2
Lys14-Gly17 1.9 ± 0.2 3.6 ± 0.4 2.3 ± 0.5 5.2 ± 1.3
Thr18-Ala23 0.9 ± 0.1 1.0 ± 0.4 1.6 ± 0.3 2.5 ± 0.8
Met24-Ser27 0.5 ± 0.1 1.7 ± 0.5 1.2 ± 0.2 2.8 ± 0.4
Asp28-Leu32 1.2 ± 0.4 N.D.a 5.2 ± 0.6 N.D.a
Glu45-Glu51 3.7 ± 0.5 N.D.a 3.5 ± 0.8 N.D.a
a

N.D. indicates that these product ions have ion intensities that are too low to accurately determine HDX rates.