Figure 7.

Effects of silencing LAMP1 on cell proliferation, migration, and invasion in PCa cell lines. (A) LAMP1 mRNA expression was determined at 72 h after transfection with si-LAMP1. GAPDH was used as an internal control. (B) LAMP1 protein expression was evaluated by western blotting at 72 h after transfection with si-LAMP1. GAPDH was used as a loading control. (C) Cell proliferation was determined by XTT assays. (D) Cell migration activity was determined by Boyden chamber migration assays. (E) Cell invasion activity was determined by Matrigel invasion assays. ^*P<0.0001. Experiments were performed in triplicate. Bars indicate SDs.