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. 2016 May 18;49(1):133–143. doi: 10.3892/ijo.2016.3531

Figure 2.

Figure 2

ImageStream high resolution flow cytometry analysis of mTOR (A), DEPTOR (B), rictor (C) and raptor (D) protein expression in MDAH-2774 cells grown under basal conditions. MDAH-2774 cells were fixed in paraformaldehyde and stained with antibodies for mTOR, DEPTOR, rictor and raptor and an Alexa Fluor® conjugated secondary antibody before visualisation. mTOR, DEPTOR, rictor and raptor were expressed at protein level in this cell line.