Figure 3.

Wound healing analysis of SKOV3 cells treated with rapamycin (Rap), resveratrol (Res) and NVP BEZ-235 (BEZ). SKOV3 cells were seeded in 6-well plates and grown to a confluent monolayer. A scratch was created in the growth area with a p200 pipette tip and the cells were cultured in media containing either Rap (20 or 100 nM), Res (25 or 50 μM) or BEZ (10 or 100 nM). The ‘wound’ was monitored over an 18-h period and images recorded at 0, 6, 12 and 18 h. mTOR pathway inhibition induced retardation of growth into the created gap.