Figure 3.

The PBOX-15/TRAIL combination significantly enhances apoptosis in ALL cells compared to either agent alone. ALL cells were treated for 24 h [Jurkat (A), CEM (B) and Nalm-6 (C)] or 48 h [Reh (D)] with vehicle [0.2% (v/v) ethanol] or PBOX-15 (1 μM) in the presence or absence of TRAIL (10–100 ng/ml). The cells were then fixed in ethanol, stained with propidium iodide and DNA content assessed by flow cytometry using CellQuest. Cells in the subG₀/G₁ phase (<2N DNA) were deemed apoptotic. Values represent the mean ± SEM for three independent experiments. A P-value <0.05 was considered to be statistically significant (^*P<0.05, ^**P<0.01).