FIGURE 5.
Dynamic quenching of fluorescence of single Trp mutants by PA or C12SL and distribution of the quenching sites in the structure of TL. (A) Dynamic quenching constants and (B) rate constants for bimolecular collisional quenching for single Trp mutants by PA (red) or C12SL (black) in TL. (A, B) ▲ and ■ symbols represent β-stand and loop regions, respectively. Single and double letters denote the identities of the β-strands and loop, respectively. (C) Ribbon representation of TL. Residues are colored according to the rate constants of dynamic quenching observed with C12SL. Inset: color coding.