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Static quenching of fluorescence of single Trp mutants by PA or C12SL and distribution of the quenching sites in the structure of TL. (A) Static quenching (or enhancement) constants for single Trp mutants by PA (red) or C12SL (black) in TL. ▲ and ■ symbols are the same as in Figure 5. (B) Solvent-accessible surface representation of TL. Residues are colored according to the static quenching constants observed with C12SL. Inset: color coding.
