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. Author manuscript; available in PMC: 2016 Jun 10.
Published in final edited form as: Biochemistry. 2009 Aug 4;48(30):7219–7228. doi: 10.1021/bi9005557

Table 3.

Fluorescence Lifetime Parameters for the Mutants W101 and W105 at Varying Concentrations of C12SL

Trp mutant C12SL (μM) α 1 α 2 τ1 (ns) τ2 (ns) τav (ns) χ 2
W101 0 0.31 0.69 0.66 4.35 4.12 1.01
2 0.35 0.65 0.97 4.36 4.00 1.04
4 0.39 0.61 1.16 4.37 3.90 0.97
8 0.39 0.61 1.12 4.28 3.83 0.94
11.9 0.46 0.54 0.98 4.06 3.54 0.91
15.6 0.56 0.44 0.93 3.93 3.24 0.99
W105 0 0.65 0.35 0.78 3.43 2.65 1.08
2 0.63 0.37 0.74 3.33 2.62 1.12
4 0.65 0.35 0.74 3.34 2.59 1.10
8 0.65 0.35 0.81 3.35 2.55 1.17
11.9 0.68 0.32 0.80 3.44 2.56 0.88
15.6 0.67 0.33 0.72 3.33 2.52 1.12