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. 2016 May 23;1(1):12–40. doi: 10.20411/pai.v1i1.72

Figure 4.

Figure 4.

Expression of HDV antigens recapitulates a Sjögren's syndrome-like phenotype in an in vivo murine model. (A) Total IgG was significantly increased in mice expressing S-HDAg and L-HDAg, but not in mice that received combination S-HDAg/L-HDAg (t-test, **P < 0.01, *P < 0.05, n = 7-15). (B) Antinuclear antibodies (ANA) were elevated in mice expressing S-HDAg (t-test, *P < 0.05, n = 6-11). (C) Anti-SSA/ Ro60 antibodies were significantly elevated in mice expressing S-HDAg, L-HDAg, and the combination of S-HDAg/L-HDAg (t-test, ***P < 0.0005, *P < 0.05, n = 7-11). (D) An increase in Anti-SSB/La antibodies was observed in mice that expressed S-HDAg (t-test, *P < 0.05, n = 6-11). (E) Mice were cannulated with adeno-associated virus (AAV) containing expression cassettes for luciferase (control), S-HDAg, L-HDAg, or a combination of the two HDV antigens (S-HDAg/L-HDAg). Mice that expressed S-HDAg showed a significant decrease in pilocarpine stimulated saliva flow compared to controls (t-test, *P < 0.05, n = 6-14). (F) A significant increase in foci was noted in mice that expressed a combination of S-HDAg and L-HDAg (t-test, **P < 0.005, n = 6-14).