Supplemental Figure 1.

HDV sequence was detected in salivary glands obtained from pSS and sicca patients. A) Nested PCR was used to detect HDV genomic sequence in minor salivary gland tissue. Sequence homology (98.5% (97.3%-99.3%)) was observed within nested PCR. B) Nested qPCR retargeted to the region encompassing HDAg tran-script and genome was used to confirm the viral sequence present in a second pSS cohort and in a selection of patients experiencing sicca symptoms but did not meet full criteria for diagnosis. Two samples identified as positive and two samples identified as negative by qPCR performed on UK co-hort were independently processed and utilized as positive and negative controls in independent confirmation of HDV by nested qPCR in additional UK co-hort.