Supplemental Figure 3.

Focal size of lymphocytic infiltrates were larger in mice expressing the combined S-HDAg and L-HDAg compared to foci size observed in mice expressing S-HDAg/L-HDAg in salivary gland tissue. A) Area of foci in mice expression a combination of both the small and large HDV antigen revealed an increase in size a subset of the observed foci (n.s., n = 7-33). There was a significant increase in variance of size in the treated mice compared to the control mice (F-test, P=0.0008). B) Lymphocytic foci representative of the average size of foci present in control mice that expressed luciferase in salivary gland tissue (2.5E-3 mm2). Average size of foci in the mice expressing S-HDAg/L-HDAg is 13.1E-3mm2 in control mice. Images of lymphocytic foci representative of the average size of foci present. C) Ectopic lymphoid structures were evident in mice expressing combined S-HDAg/L-HDAg. Distribution of T-cell CD3 (green) and B-cell B220 (red) depict transition to organized lymphoid-like structures (bar 100um)