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. Author manuscript; available in PMC: 2016 Jun 10.
Published in final edited form as: Int J Transl Sci. 2016 Jan;2016(1):5–32. doi: 10.13052/ijts2246-8765.2016.002

Figure 1.

Figure 1

Figure 1

Prelabeled MSCs and Keratinocytes in vivo mimic the organization as observed in vitro. Prelabeled MSCs (CFDA-SE; Green) and Keratinocytes (DIL; Red) (1:5) were cocultured for 4 days. MSCs appear to encircle the keratinocytes in vitro(a) and when transplanted locally at wound site. CFDA-SE labeled MSCs again appear to encircle the red DIL labeled keratinocytes (d–f). Whereas other similar cell types when labeled and co cultured together as a control i.e. (b) WI38 (green) with keratinocyte (red), (c) MSC (green) with MDAMB 231 (red) were not able to follow the same construction. Snap frozen wounded skin sections were cut and counter stained with DAPI. (d) DIL labeled (red) keratinocytes. (e) Green MSC circle formation; Inset shows single CFDA-SE labeled hMSC. (f) Section counterstained with DAPI (blue) and merged; merged images (green, red and blue) clearly show green MSCs surrounding the red keratinocytes. (g) Merge confocal image of KCMSCs stained for vinculin (green) and phalloidin (red). The focal adhesions (green) appear to hold down actin stress fibers (red). Inset shows actin filaments terminating with vinculin at the cell periphery. (h) KGMSCs showing diffused vinculin staining when compared with KCMSCs. (i) Naïve hMSCs stained for vinculin and phalloidin as a control. (j) Differentiated KCMSCs and KGMSCs stained for α-SMA, (k) FSP and (l) Vimentin (m). Graph showing Quantitative analysis of KCMSC and KGMSC expressing various markers: α-SMA, FSP and vimentin. Scale bars, (a–c) 100 µm, (d–f) 50 µm, (g–i) 500 µm, (j–l) 300 µm.