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. 2016 Jun 10;11(6):e0157251. doi: 10.1371/journal.pone.0157251

Fig 1. Effect of individual or combined RQC on Akt activity in breast cancer cells.

Fig 1

Quiescent MDA-MB-231 cells were treated with vehicle, or 1, 3, 5, 9, or 15μM of resveratrol (Res), quercetin (Quer), catechin (Cat), or combined Res, Quer, and Cat (RQC) at 3μM total (1μM each), 9μM total (3μM each), or 15μM total (5μM each). MDA-MB-435 cells were treated only with vehicle or 15μM quercetin. Cells were lysed immediately following treatment for 15min, and western blotted for total or active (phospho-AktSer473) Akt. (A) Relative Akt activity (phospho-Akt/Akt) of MDA-MB-231 cells following RQC or individual Res, Quer, or Cat at the indicated concentrations. (B) Relative Akt activity of MDA-MB-231 cells following vehicle or 15μM quercetin. (C) Relative Akt activity of MDA-MB-435 cells following vehicle or 15μM quercetin. For (B) and (C), representative western blots from 3 separate experiments are shown. Graphs show the analyses of the integrated densities of positive bands relative to vehicle, as quantified from image J analysis. An asterisk indicates statistical significance (p≤0.05) when compared to vehicle.