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. 2016 Jun 10;11(6):e0157317. doi: 10.1371/journal.pone.0157317

Fig 3. Overexpression of Nupr1 reduces both H4K16ac and histone acetyltransferase MOF and increases H3K4me3.

Fig 3

(A) BEAS2B cells were transfected with Nupr1 expression vector or empty (Crtl) vector. The control cells were treated with or without Cr(VI) for 24 hours. The cell lysates were prepared with RIPA buffer, and run on a 14% SDS acrylamide gel. Western blot of Nupr1, H4K16ac, MOF, a number of other histone modifications and ß-actin was represented. Band intensities were measured by ImageJ software. (B and C) Total RNA was extracted either from BEAS2B cells that overexpress Nupr1 (B) or from Nupr1 knockdown cells (C). MOF and Nupr1 mRNA levels were measured by RT-qPCR and normalized to γ-tubulin. The transcription level was presented as fold change compared to the control group. The data shown are the mean ± S.D. from qPCRs performed in triplicate. *, p<0.01.