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. 2016 Jun 10;11(6):e0157317. doi: 10.1371/journal.pone.0157317

Fig 5. Reduction of H4K16ac induced by overexpression of Nupr1 correlates with transcriptional inactivation.

Fig 5

(A) Levels of H4K16ac are downregulated at several genomic loci by overexpression of Nupr1. Mono- and dinucleosomes were prepared by MNase digestion from BEAS2B cells transfected with Nupr1 plasmid (pCMV-Nupr1) or empty vector (Ctrl) and subjected to ChIP assays with antibodies specific for H4K16ac. The enrichment of H4K16ac was measured by qPCR at several genomic loci including promoters of IAP (inhibitor of apoptosis), TRIM42S (tripartite motif-containing 42), and D4Z4 subtelomeric region. The data shown are presented as mean ± S.D. from qPCRs performed in triplicate. Relative-fold changes were calculated after normalization to input. *, p<0.05; **, p<0.01. (B) RT-qPCR measurements of transcripts in BEAS2B cells transfected with Nupr1 plasmid (pCMV-pur1) or empty vector (Ctrl). The amount of mRNA in control cells was set to 1. The data shown are the mean ± S.D. (error bars) from qPCRs performed in triplicate. *p<0.05; ** p<0.01.