Skip to main content
. 2016 May 3;3(2):e38. doi: 10.14440/jbm.2016.102

Figure 2.

Figure 2.

Total protein tracking and capture through centrifugations and final pellet suspension. Fractions collected as 1.0 ml aliquots. Aqueous layer A is PEG/NaCl treated supernatant immediately following 3000 × g centrifugation. Crude Suspension represents the PEG/NaCl pellet resuspended in 50 ml 250 mM NaCl prior to 10,000 × g centrifugation. Aqueous Layer B represents the suspension buffer immediately following the final 149,000 × g centrifugation. AAV pellet is suspended in 7.0 ml 250 mM NaCl.