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SDS-PAGE electrophoresis on 20 microliter aliquots taken at different stages of purification. Lane: (1) Marker (Bio-Rad Precision Plus), (2) Crude supernatant pre-enzymatic digest, (3) Crude supernatant post filtration, (4) PEG/NaCl treated supernatant pre-3,000 × g centrifugation, (5) Aqueous layer post 3,000 × g centrifugation, (6) Crude PEG/NaCl pellet suspended in 40 ml Suspension Buffer pre-10,000 × g spin, (7) Virus containing aqueous layer post 10,000 × g spin.
