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SDS-PAGE electrophoresis of purified and suspended AAV2/6. Lane: (M) Marker (Bio-Rad Precision Plus), (1) Viral pellet post 149,000 × g centrifugation suspended in 10ml buffer, (2) AAV2/6 viral suspension dialyzed overnight in suspension buffer to demonstrate further purification steps are not required and may result in titer loss, (3) heparin column purified AAV2/6 control, space indicates extraneous wells removed.
