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. Author manuscript; available in PMC: 2017 Jun 6.
Published in final edited form as: Dev Cell. 2016 May 26;37(5):399–412. doi: 10.1016/j.devcel.2016.05.002

Figure 2. SnoN regulates TAZ levels in both mammary tissues and epithelial cell lines.

Figure 2

(A) Total proteins from pregnancy day 18.5 SnoN+/+ and SnoN−/− mammary glands were subjected to Western blotting analyses. Smad2 was used as a loading control. (B) Immunohistochemistry staining of TAZ in pregnancy day 18.5 SnoN+/+ and SnoN−/− mammary sections. Boxed area highlighted the TAZ staining in ductal epithelial cells. Bar, 100 μm. (C) Lysates from cells transfected with a fixed amount of Flag-TAZ (F-TAZ) and increasing amounts (0, 0.5, 1, 2 μg) of Flag-SnoN (F-SnoN) were assessed by Western blotting with anti-Flag. Tubulin was used as a loading control. (D) TAZ and CTGF expression in parental and shSnoN MCF10A cells was analyzed by Western blotting. (E) Reducing TAZ by siTAZ did not affect SnoN levels as assessed by Western blotting. (F) MCF10A cells stably expressing shSnoN or scramble control were treated with 70 μM H2O2 for 24 h, and levels of SnoN and TAZ analyzed by Western blotting. (G) Cells were stimulated with 100 pM TGFβ for the indicated time, and levels of SnoN and TAZ analyzed by Western blotting.