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. Author manuscript; available in PMC: 2017 Jun 6.
Published in final edited form as: Dev Cell. 2016 May 26;37(5):399–412. doi: 10.1016/j.devcel.2016.05.002

Figure 3. SnoN promotes TAZ stability.

Figure 3

(A) Parental MCF10A cells or those stably expressing F-SnoN were treated with 100 μg/ml cycloheximide (CHX) for the indicated times. TAZ levels were measured by Western blotting with anti-TAZ and quantified in the graph shown to the right. (B) MEFs expressing scramble control or shSnoN were subjected to CHX treatment as described above in A. (C) TAZ level was measured in lysates prepared from MCF10A cells expressing shSnoN or scramble control after treatment with or without 20 μM MG132 for 6 h. (D) mSnoN enhanced TAZ expression as efficiently as WT SnoN as shown by Western blotting with anti-TAZ and anti-Flag, respectively.

See also Figure S2A&B.