Figure 2.

Stopped-flow data of Lti30 binding and clustering of PC:PG lipid vesicles (LUVs). Lti30 at 3.5 μm was rapidly mixed with 0.5 mm PC:PG vesicles at a 1:142 protein:lipid ratio. Binding of positively charged Lti30 promotes vesicle clustering, monitored by scattering at 400 nm. The process depends on the LUV net negative charge, which was varied between PG (1%–40%) and PC (99%–60%). A, Scattering at 400 nm. B, Absorbance at 400 nm. C, Scattering amplitude at 400 nm at 0.5 s, indicating two binding modes.