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. 2016 Apr 26;171(2):932–943. doi: 10.1104/pp.15.01531

Figure 4.

Figure 4.

Vesicle clustering by titration of various K-segments correlates to peptide net charge. Clustering of lipid vesicles was measured by absorbance at 400nm and peptide net charge was calculated at http://protcalc.sourceforge.net. A, Titration of different K-segments into 0.5 mM PC:PG vesicles (1:3) at pH 6.3 (blue lines) and pH 4.3 (red lines). The vesicle clustering follows peptide net charge. KLti30 represent the dominating type of K-segment in Lti30, KC is the consensus K-segment and KHHCHH is KC with the addition of histidine flanks. KC fail to bind vesicles regardless of pH, showing the regulatory impact of positively charged histidine flanks. B, Peptide sequences, with positively and negative residues in blue and red, respectively.