Figure 7.

Atomic resolution structure of the membrane-binding domain of Lti30. A to C, Atomic model of the membrane-binding K^Lti30 segment of Lti30, in side, diagonal, and front views, respectively. Positively charged residues are in blue, negatively charged residues are in red, and the hydrophobic side of the amphipathic α-helix faces downward. D, NMR diffusion data of K^Lti30 alone, bicelles alone, and K^Lti30 mixed with bicelles, verifying K^Lti30 micelle binding. a.u., Arbitrary units. E, Model of the positioning of the His-flanked K^Lti30 segment into a lipid bilayer (PC:PG 98:2 mix), using periodic boundary conditions. Consistent with the binding data in Figures 2 to 5, the positive side chains H2, H3, K5, and K6 coordinate the negative charge of a PG head group.