Skip to main content
. 2016 Apr 18;171(2):864–877. doi: 10.1104/pp.15.01796

Figure 3.

Figure 3.

Accumulation and assembly of NDH-1L and NDH-1M complexes from the thylakoid membrane of wild-type (WT), ∆ssl3829, ∆slr1097, and M55 strains. A, Immunodetection of Ndh subunits in thylakoid membranes from the wild type (including the indicated serial dilutions) and ∆ssl3829, ∆slr1097, and M55 mutants. Immunoblotting was performed with antibodies against hydrophilic Ndh subunits (NdhH, NdhI, NdhK, and NdhM). Lanes were loaded with thylakoid membrane proteins corresponding to 1 µg of Chl a (100%). At bottom, PsaD was used as a loading control. B, Thylakoid protein complexes isolated from the wild type and mutants were separated by BN-PAGE. Thylakoid membrane extract corresponding to 9 µg of Chl a was loaded onto each lane. The positions of NDH-1L and NDH-1M complexes are indicated by red and blue arrows, respectively. C, Protein complexes were electroblotted to a polyvinylidene difluoride membrane, and the membrane was cross-reacted with anti-NdhH, anti-NdhI, anti-NdhK, and anti-NdhM to probe the assembly of NDH-1L and NDH-1M complexes.