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Localization of Ssl3829. The proteins of soluble cytoplasm and total membrane were isolated from the WT-Ssl3829-YH strain, and immunoblot analyses were performed with the indicated antibodies. Cytoplasmic and membrane proteins (10 µg) were loaded on each well (100%). Serial dilutions of cytoplasmic proteins are indicated, and detection of RbcL was used as the loading control. SF and MF represent soluble and total membrane fractions, respectively.
