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. 2016 Apr 18;171(2):864–877. doi: 10.1104/pp.15.01796

Figure 8.

Figure 8.

Relationship between Ssl3829 and Slr1097. A, Western analysis of Slr1097 from the wild-type (WT; including indicated serial dilutions) and ∆ssl3829 strains. Lanes were loaded with the cytoplasmic proteins corresponding to 50 µg (100%). RbcL was used as a loading control. B, RT-PCR analysis of slr1097 from the wild-type and ∆ssl3829 strains. The transcript level of 16 S rRNA in each sample is shown as a control. The absence of contamination of DNA was confirmed by PCR without reverse transcriptase. C, PCR segregation analysis of ssl3829-yfp-his6 from the wild-type and Δslr1097-Ssl3829-YH strains using the ssl3829-yfp-his6-E and -F primer sequences (Supplemental Table S1). D, Western analysis of proteins from the wild-type and Δslr1097-Ssl3829-YH strains using GFP and His antibodies. Total protein corresponding to 1 µg of Chl a was loaded onto each lane, and RbcL was detected as a loading control. E, Western analysis of Ssl3829 from the wild-type and Δslr1097 strains having Ssl3829-YH. Lanes were loaded with the cytoplasmic proteins corresponding to 50 µg (100%). RbcL was used as a loading control.