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. 2016 Apr 4;171(2):821–832. doi: 10.1104/pp.15.01878

Figure 4.

Figure 4.

NAB1 is nitrosylated in vivo at Cys-226 under low-light conditions. C. reinhardtii cells overexpressing wt-NAB1 or NAB1C226S were grown under low light (40 μmol m−2 s−1) or elevated light (200 μmol m−2 s−1) for 8 h and then subjected to BST. After BST, biotinylated proteins were purified by streptavidin affinity chromatography and eluted with DTT. Eluted proteins were analyzed by western blot using αNAB1 antibodies (NAB1-SNO). Immunoblot detection of NAB1 before chromatography (NAB1 total) served as control. Bands shown in one panel derive from the same blot and were rearranged for clarity.