Figure 2.

Mutants with reduced ILK1 expression exhibit reduced root sensitivity to bacterial flg22. A, ILK1 gene expression is reduced in ilk1-1 and ilk1-2 T-DNA insertion lines. Expression was quantified by qRT PCR in 4–6 biological replicates with two technical replicates and normalized to protein phosphatase 2A (PP2A) gene expression where * indicates significant difference from the wild type, P < 0.05. B, Flg22-induced root growth inhibition is absent in ilk1-1 and ilk1-2 seedlings. The seedlings were grown for 5 d prior to transfer to liquid MS media containing no flg22 or 100 nm flg22 and primary root length was quantified 10 d after transfer (n = 10–16) where * indicates significant difference in growth between the flg22-exposed and non-exposed plants within a line, P < 0.05.