Skip to main content
. 2016 May 2;171(2):1470–1484. doi: 10.1104/pp.16.00035

Figure 4.

Figure 4.

Some flg22-induced signaling responses are altered in ilk1-1 plants. A, Flg22-induced ROS production is intact in ilk1-1 plants. Leaf disks were exposed to 100 nm flg22 and ROS was quantified in relative light units immediately after exposure. Nine to 12 plants were measured in duplicate and the experiment was repeated three times. B, MAPK activation dynamics are altered in ilk1-1. Three to 5 seedlings were treated with 1 μm flg22 and harvested at the indicated time after exposure. MAPK phosphorylation was monitored by western blot with antip44/42 MAPK antibody. Ponceau staining is shown to assess equal loading. C, PAMP-triggered immunity-induced gene expression is enhanced in ilk1-1 and ILK1K222A-expressing seedlings. Two marker genes downstream of MAPK activation were evaluated for induction after treatment with 1 μm flg22 for 2 h compared to untreated seedlings. FRK1 and WRKY29 expression were quantified by qRT PCR in five biological replicates with two technical replicates and normalized to PP2A gene expression where * indicates significant difference from the wild type, P < 0.05.