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. 2016 Apr 18;171(2):1511–1522. doi: 10.1104/pp.16.00057

Figure 4.

Figure 4.

Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays for detecting interacting partners of PYL1 or ABI5. A, Two-hybrid validation in yeast of the interactions between PYL1 and ARR1 (AT3G16857) as well as between ABI5 and TGA5 (AT5G06960), ELIP (AT3G22840), RAV1 (AT1G13260), and DDF1 (AT1G12610). BD-protein indicates the fusion protein with the Gal4 binding domain, and AD-protein indicates the fusion protein with the Gal4 activation domain. AD or BD was used as a negative control. +His indicates synthetic dropout medium deficient in Leu and Trp, and −His indicates synthetic dropout medium deficient in Leu, Trp, and His. B, BiFC assay for ABI5 and RAV1. 35S:ABI5-YFPN and 35S:RAV1-YFPC constructs were coinfiltrated into N. benthamiana leaves. YFP signal intensity was detected from 48 to 60 h after infiltration.