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. 2016 May 2;171(2):1259–1276. doi: 10.1104/pp.16.00059

Figure 2.

Figure 2.

OsDET1 deficiency accelerates dark-induced leaf senescence in OsDET1 RNAi plants. A, qRT-PCR analysis of the expression of OsDET1 in OsDET1 RNAi lines and the wild type (WT). B, Suppression of OsDET1 promoted dark-induced leaf senescence. C, Changes with time of chlorophyll content in OsDET1 RNAi lines and wild-type plants during dark incubation. Values are means ± sd of nine measurements. The penultimate leaves were detached and incubated with water in darkness at the tillering stage. FW, Fresh weight. D, Changes with time of Fv/Fm values in OsDET1 RNAi lines and wild-type plants during dark incubation. Values are means ± sd of nine measurements. E, Changes with time of membrane ion leakage in OsDET1 RNAi lines and wild-type plants during dark incubation. Values are means ± sd of nine measurements. F to H, Expression of NOL, SGR, and OsNAP in the wild type and OsDET1 RNAi lines during dark incubation. **, P ≤ 0.01 (Student’s t test).