OsDET1 influences seed germination and seedling growth. A, Overexpression or suppression of OsDET1 inhibited seed germination in transgenic plants. B, Germination time courses on one-half-strength MS medium. C, Overexpression or suppression of OsDET1 inhibited seed germination in transgenic plants during ABA treatment. D, Germination time courses on one-half-strength MS medium containing 2 µm
ABA. E, Overexpression or suppression of OsDET1 inhibited shoot growth and promoted root growth. F, Overexpression or suppression of OsDET1 inhibited the growth of shoot and root during ABA treatment. G and H, Root and shoot growth rates of the wild type (WT) and OsDET1 RNAi and OE-OsDET1 plants. Three independent experiments were carried out with similar results. Representative graphs are shown (n = 30 seeds in each experiment). *, P ≤ 0.05 and **, P ≤ 0.01 (Student’s t test).