Figure 6.

Interaction of OsDET1, OsDDB1, OsCOP10, OsDDA1, and OsPYL5 in vivo. A, OsDET1 interacts with OsDDB1 and OsCOP10 in yeast cells. The coding range of OsDET1 was inserted into pGBKT7 as bait, and the coding ranges of OsDDB1 and OsCOP10 were inserted into pGADT7 as prey. B, OsDDA1 interacts with OsDDB1 and OsPYL5 in yeast cells. The coding range of OsDDA1was inserted into pGBKT7 as bait, and the full-length coding sequence of OsDDB1 and a truncated version of the ABA receptor OsPYL5 (amino acids 90–209) were fused separately to pGADT7 as prey. AbA, Aureobasidin A; 3-AT, 3-amino-1,2,4-triazole; SD, synthetic dextrose. C, OsDET1 interacts with OsDDB1 and OsCOP10 in vivo. YFP^N-OsDET1/YFP^C-OsDDB1 and YFP^N-OsDET1/YFP^C-OsCOP10 were transformed into onion epidermal cells by Agrobacterium tumefaciens-mediated transformation to test their interactions. After 48 h of incubation in the dark, YFP signal was detected by confocal microscopy. Bright field was used to indicate the localization of nuclei. Empty vectors were used as negative controls. D, OsDDA1 interacts with OsDDB1 and OsPYL5 in vivo. YFP^N-OsDDA1/YFP^C-OsDDB1 and YFP^N-OsDDA1/YFP^C-OsPYL5 were transformed into onion epidermal cells by A. tumefaciens-mediated transformation to test their interactions.