Figure 7.

OsDET1 influences the degradation of OsPYL5 and activates the expression of ABA mark genes. Fourteen-day-old seedlings were treated with ABA solution (one-half-strength MS medium with 5 μm ABA) for 12 h. Shoots were detected by qRT-PCR. A to D, Expression of OsPYL5, OsRAB16, OsLIP9, and OsLEA3 in the wild type (WT) and OsDET1 RNAi lines during ABA treatment. **, P ≤ 0.01 (Student’s t test). E, Western-blot analysis of OsPYL5 from detached OsDET1-GFP leaves at the tillering stage after 12 h of ABA treatment. F, Western-blot analysis of OsPYL5 from detached OE-OsDET1 leaves at the tillering stage after 12 h of ABA treatment. G, Western-blot analysis of OsPYL5 from detached OsDET1 RNAi leaves after 12 h of ABA treatment.