Figure 7.
Effect of different nitrogen form (NH4+ or NO3−) on the expression of Pi translocation-related genes. A, B and C, ‘Nip’ cultivar; D, E and F, ‘Kas’ cultivar. Seedlings were transferred to P-deficient (−P) or P-sufficient (+P) nutrient solution in the presence of NH4+ or NO3− for 1 week. After treatment, total root RNA was extracted and used to synthesize cDNA. Quantitative RT-PCR was performed using gene-specific primers. Data are means ± sd (n = 4). Columns with different letters are significantly different at P < 0.05.