Table I. Overview of the GAL4/UAS-GFP expression patterns and GAL4 insertion positions.

n.d., Not detected; RAM, root apical meristem.

Driver Line/ARBC Seed Stock No.	Expression Pattern			Insertion Position (from the ATG)	Flanking Gene(s)	Chromosomal Position	Strand	Short Gene Description	Reference
	Embryo	Root	Other
Group 1: Early embryo development lines
Q0990/N9217	Provascular cells	Vasculature	n.d.	−15,383 bp	AT5G43810	17,596,556	Forward	Encodes AGO10, a member of the EIF2C (elongation initiation factor 2c)/Argonaute class of proteins
				−823 bp	AT5G43800		Forward	Transposable element gene
				−3,074 bp	AT5G43790		Complement	Pentatricopeptide repeat superfamily protein
Q2500/N9135	Ground tissue, mesophyll precursors, protoderm of cotyledons	Ground tissue, pericycle, and QC	Cotyledon junction and guard mother cells	+1,105 bp	AT4G02590	1,139,201	Complement	Unfertilized embryo sac 12 (UNE12), bHLH transcription factor
				−4,939 bp	AT4G02600		Forward	MLO1; a member of a large family of seven-transmembrane domain proteins specific to plants, homologs of the barley mildew resistance locus o (MLO) protein
Group 2: Late embryo development lines
J0571/N9094	Ground tissue, basal protoderm cells	Ground tissue, some QC cells	Guard and pavement cells	−732 bp	AT4G39900	18,511,021	Complement	Unknown protein	Miyashima et al. (2011)
					AT4G39910		Complement	Encodes a nuclear ubiquitin-specific protease
J2731	Vascular tissue	Cells above QC	Guard and pavement cells	−2,418 bp	AT1G21040	7,365,752	Complement	Transposable element gene	Møller et al. (2009)
				−1,106 bp	AT1G21050		Forward	Unknown protein
M0148/N9303	Cells surrounding SAM	Pericycle cells	Cells surrounding SAM	−2,369 bp	AT1G78300	29,459,514	Forward	G-box binding factor GF14 omega encoding a 14-3-3 protein
				−604 bp	AT1G78290		Complement	Encodes a member of SNF1-related protein kinase (SnRK2) family whose activity is activated by ionic (salt) and nonionic (mannitol) osmotic stress and dehydration
M0164/N9307	Vascular strands in embryonic cotyledon primordia	Pericycle	Shoot vasculature	−4,452 bp	AT1G09520	3,076,482	Complement	Domains: zinc-finger, PHD-type, conserved site
				−733 bp	AT1G09530		Forward	(PIF3) Transcription factor interacting with photoreceptors phyA and phyB
				−1,763 bp	AT4G00440	193,413	Forward	TON1 RECRUITING MOTIF15 (TRM15)
				−5,881 bp	AT4G00430		Complement	A member of the plasma membrane intrinsic protein subfamily PIP1
M0167/N9308	Intersection of the two cotyledon primordia	n.d.	Cotyledon junction	−4,452 bp	AT1G09520	3,076,482	Complement	Domains: zinc-finger, PHD-type, conserved site
				−733 bp	AT1G09530		Forward	(PIF3) Transcription factor interacting with photoreceptors phyA and phyB
M0223/N9336	Intersection of the two cotyledon primordia	n.d.	Cotyledon junction	−4,461 bp	AT3G15150	5,108,543	Complement	Encodes a SUMO E3 ligase that regulates endocycle onset and meristem maintenance	Cary et al. (2002)
				−264 bp/−1,368 bp	AT3G15160/AT3G15170		Forward	Unknown protein/CUC1, encodes a transcription factor involved in SAM formation and auxin-mediated lateral root formation
Group 3: Embryonic RAM lines
J1092/N9147	Future RAM and LRC cells	RAM, LRC	n. d.	+81 bp	AT2G28400	12,148,920	Complement	Unknown protein
				−1,459 bp	AT2G28401		Forward	Unknown protein
J3281/N9128	Future RAM	Young vascular tissue; columella cells	n.d.	−6,847 bp	AT5G62230	24,989,586	Forward	Encodes a receptor-like kinase that, together with ER and ERL2 governs the initial decision of protodermal cells to either divide proliferatively to produce pavement cells or divide asymmetrically to generate stomatal complexes
				+424 bp	AT5G62220		Complement	Encodes a Golgi apparatus-localized galactosyltransferase involved in galactosyl-substitution of xyloglucan at position 2
J3411/N9131	Future RAM and LRC cells	RAM, LRC	n. d.	−5,801 bp	AT2G36360	15,253,324	Complement	Gal oxidase/kelch repeat superfamily protein
					AT2G36370/80		Forward	Ubiquitin-protein ligases/pleiotropic drug resistance 6 (PDR6)
M0028/N9274	Future RAM	Columella cells	n.d.	−1,463 bp	AT4G21690	11,525,766	Forward	Gibberellin 3-oxidase 3 (GA3OX3)
				−6,189 bp	AT4G21680		Complement	Encodes a nitrate transporter (NRT1.8); functions in nitrate removal from the xylem sap
Q0680/N9209	Future RAM	Columella cells	n.d.	−1,749 bp	AT2G45200	18,641,389	Complement	Encodes a member of the GOS1 (Golgi SNARE) gene family
				−494 bp	AT2G45210		Forward	SAUR36,SAUR-like auxin-responsive protein family
Q1630/N9227	Future RAM	Columella cells	n.d.	−1,749 bp	AT2G45200	18,641,389	Complement	Encodes a member of the GOS1 (Golgi SNARE) gene family
				−494 bp	AT2G45210		Forward	SAUR36,SAUR-like auxin-responsive protein family
Other lines
J0121/N9090	Seed Coat	Xylem pole pericycle cells	Young leaf primordia	−3,701 bp	AT5G01750	286,333	Forward	Unknown protein	Laplaze et al. (2005)
				−1,253 bp	AT5G01747		Forward	Encodes a microRNA that targets several genes containing NAC domains including NAC1
				−5,051 bp	AT5G01740		Forward	Nuclear transport factor 2 (NTF2) family protein
Q0171/N9207	n. d.	Root cap	n.d.	−9,611 bp	AT1G04690	1,304,051	Forward	Potassium channel β-subunit 1 (KAB1)
					AT1G04680		Complement	Pectin lyase-like superfamily protein
RM1000	n. d.	Leaf margins	n.d.	−649 bp	AT2G42010	17,538,639	Complement	Phospholipase D (PLDβ)
					AT2G42030		Complement	RING/U-box superfamily protein
No expression lines
J2501/N9121	n. d.	n.d.	n.d.	−4,314 bp	AT1G17744	6,106,109	Complement	Unknown protein
					AT1G17745/50		Forward	Encodes a 3-phosphoglycerate dehydrogenase/encodes PEPR2, a plasma membrane Leu-rich repeat receptor kinase functioning as a receptor for the Pep1 and Pep2 peptides
J2661/N9187	n. d.	n.d.	n.d.	−129 bp	AT2G01010	3,577	Forward	18S rRNA
					AT2G01008		Forward	Unknown protein
				−129 bp	AT3G41768	14,197,548	Forward	18S rRNA
					AT3G41761		Forward	Other RNA
M0136/N9302	n. d.	n.d.	n.d.	−3,605 bp	AT1G32140	11,568,438	Complement	F-box family protein
					AT1G32150/60		Forward	Encodes a G group bZIP(68) transcription factor family member that can bind cis-elements with an ACGT core, such as G-box, Hex, C-box, and As-1/unknown protein