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. 2016 Apr 4;171(2):1037–1054. doi: 10.1104/pp.16.00230

Figure 7.

Figure 7.

Symbiotic gene expression is either down- or up-regulated in the ern1-1 ern2-1 double mutant. QRT-PCR analyses of ENOD11, ENOD12, VPY, RPG, and NIN transcripts was performed using total RNA samples extracted from wild type A17S, ern2-1, ern1-1, and the double ern1-1 ern2-1 mutant after 6 h treatment with either water control (−) or 10−9 m NF solution (+). Values represent the average from RNA samples from pooled plants (n = 26 A17S, n = 24 ern2-1, n = 35 ern1-1, and n = 35 ern1-1 ern2-1) from three to five independent biological experiments after normalization against reference transcript levels. Box plots represent first and third quartile (horizontal box sides), and minimum and maximum (outside whiskers). Black circles depict mean values. One-way ANOVA followed by a Tukey HSD test of the values was performed (in A, B, D, and E, P < 0.001; in C, P < 0.01). Classes sharing the same letter are not significantly different.